A three-part series of laboratory activities is presented that allows students to learn why electrophoretic separations work and to manipulate the factors that influence the separation process. In the first two exercises, students perform cooperative separations of FD&C dyes from candies utilizing electrophoresis in two buffers of different pH. Students must use package information from one brand of candy and critical thinking to determine the identities of these dyes. Using structural formulas for the dyes the students draw conclusions regarding the effect of charge and molecular mass on separation. The final activity is an open-ended investigation into a student-formulated question. These activities integrate topics in biology and chemistry into activities that interest students and foster a real understanding of the tool of electrophoresis.
Supplement
Student handouts and instructor notes are available as supplemental material.
Contents
JCE2000p1323Wfigures.doc (Microsoft Word 98, Macintosh), JCE2000p1323Wstudent.doc (Microsoft Word 98, Macintosh), JCE2000p1323Wteacher.doc (Microsoft Word 98, Macintosh), F0190878.pic (Photoshop PICT, Macintosh), F0290878.cdr (ChemDraw), F0390878.cdr (ChemDraw), F0490878.pic (Photoshop PICT, Macintosh)
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Through special arrangement with the ACS, JCE High School CLIC is now able to provide subscribers with online access to Chemical & Engineering News articles that have been selected specifically for secondary science instructors and their students.
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