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  Home > JCE Print > Journal of Chemical Education > Issues > 2006  > April  >
In the Laboratory
Cost-Effective Teacher
Demonstration of Absorbance Using Digital Color Image Analysis and Colored Solutions
Shane K. Kohl, James D. Landmark, and Douglas F. Stickle
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-3135
Cover
April 2006
Vol. 83 No. 4
p. 644
Abstract
This article describes a simple experiment in which the principle of absorbance may be demonstrated using digital color image analysis. Dilute yellow food coloring solutions in water were prepared in known relative concentrations and photographed in transparent cuvettes as a group placed against a diffuse fluorescent white light lightbox. Image analysis of the intensity of the complementary color (blue) for each solution produced data that conformed to the Beer–Lambert law.
More Information
*  Citation
 Kohl, Shane K.; Landmark, James D.; Stickle, Douglas F. J. Chem. Educ. 2006 83 644.
*  Keywords
 Analytical Chemistry; Aqueous Solution Chemistry; Dyes / Pigments; First-Year Undergraduate / General; Hands-On Learning / Manipulatives; High School / Introductory Chemistry; Laboratory Computing / Interfacing; Laboratory Instruction; Quantitative Analysis; Solutions / Solvents; Spectroscopy; UV-Vis Spectroscopy
*  History
 Created:
Last Updated:		 2/24/2006
2/24/2006
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