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  Home > JCE Print > Journal of Chemical Education > Issues > 1997  > September  >
In the Laboratory
The Sweetness of Aspartame: A Biochemistry Lab for Health Science Chemistry Courses
Paul J. Stein
College of Scholastica, Department of Chemistry, 1200 Kenwood Ave., Duluth, MN 55811-4199
Cover
September 1997
Vol. 74 No. 9
p. 1112
Abstract
A laboratory exercise for Health Science Biochemistry students to study the effect of aspartame concentration on sweetness has been developed. The concentration dependence of the absorbance of aspartame at 257 nm is also studied. Data from all members of the class are averaged and plotted on the same graph as absorbance and taste rating vs. [aspartame]. The absorbance plot follows Beer's law while the taste rating plot displays the typical hyperbolic response of protein-ligand binding plots. This laboratory exercise illustrates the concept of binding saturation to students.

See Letter re: this article.
More Information
*  Citation
 Stein, Paul J. J. Chem. Educ. 1997 74 1112.
*  Keywords
 Laboratory, Food Science, Non-major courses, Proteins, and Biochemistry
*  History
 Created:
Last Updated:		 July 28, 1999
June 23, 2005
Link to Letter added (April 2004).
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