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1999
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February
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In the Laboratory
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Student Understanding of Chromatography: A Hands-On Approach
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Robert D. Curtright
Lincoln Northeast High School, 2635 North 63rd St., Lincoln, NE 68507
Randy Emry
Lincoln Southeast High School, 2930 South 37th St., Lincoln, NE 68506
John Markwell
Department of Biochemistry, University of Nebraska, Lincoln, NE 68588-0664
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February 1999
Vol. 76 No. 2
p. 249
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| Abstract |
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This exercise sets up a collaborative activity that challenges students to develop a chromatographic solvent with the appropriate polarity to separate leaf chlorophyll and anthocyanin pigments by TLC. The suggested activity has been tested with both high-school advanced-chemistry students and with undergraduate students in an introductory biochemistry class. It includes a demonstration introducing the basic principles of partitioning and differential distribution based on solubility. For the student activity, the materials to be used are leaves of the variegated Coleus plant, plastic-backed silica TLC plates, 2-propanol, acetone, and methanol. Students may work collaboratively within a set of boundary parameters to optimize the chromatographic solvent through a series of rapid iterations of chromatography. Students can also use these chromatography systems to carry out independent projects.
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| More Information |
 Citation |
Curtright, Robert D.; Emry, Randy; Markwell, John. J. Chem. Educ. 1999 76 249.
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Keywords |
Biochemistry; Laboratory Instruction; Chromatography; Natural Products; Separation Science; Undergraduate Research
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History |
Created:
Last Updated: |
June 15, 1999
June 22, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
1999
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February
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249
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