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  Home > JCE Print > Journal of Chemical Education > Issues > 1999  > May  >
In the Laboratory
Concepts in Biochemistry
Synthesis and Purification of a Hammerhead Ribozyme and a Fluorescein-Labeled RNA Substrate. A Biochemistry Laboratory: Part 1
Christine S. Chow and Smita Somne
Department of Chemistry, Wayne State University, Detroit, MI 48202
Cover
May 1999
Vol. 76 No. 5
p. 648
Abstract
The applications of in vitro transcription and chemical synthesis of RNA are discussed. This laboratory describes the in vitro synthesis of a 38-nucleotide hammerhead ribozyme and the synthesis of a 17-nucleotide fluorescein-labeled RNA substrate by using standard phosphoramidite methodologies, two widely used methods in modern RNA research. The synthesis and purification procedures outlined allow students to develop an understanding of RNA handling procedures, synthesis of modified nucleic acids, gel electrophoresis, visualization of RNA by nonradioactive techniques, and quantitation of nucleic acids. The RNAs that are synthesized have applications in biotechnology and medicine; thus the students gain access to current problems in chemical and clinical research.
Supplement
A student handout and the instructor's notes contain a detailed version of the laboratory experiment. The material can be downloaded as a pdf file using Acrobat Reader. The material is also available as a Microsoft Word document, which has been compressed into both a sit file (for Macintosh) and a zip file (for Windows).
*  Contents
*  Download
supp648.pdf

supp648.zip

supp648.sit
More Information
*  Citation
 Chow, Christine S.; Somne, Smita. J. Chem. Educ. 1999 76 648.
*  Keywords
 Biochemistry; Bioorganic Chemistry; Electrophoresis; Nucleic Acids / DNA / RNA; Enzymes; Bioinorganic Chemistry
*  History
 Created:
Last Updated:		 June 11, 1999
November 22, 2005
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