JCE Online Journal of Chemical EducationDivision of Chemical Education, American Chemical SocietyAmerican Chemical Society
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print
   Current Issue
 Previous Issues
 Supplements
 Search JCE Index

JCE Digital Library
   ChemInfo
 DigiDemos
 Featured Molecules
 LivTexts
 LrnCom
 QBank
 SymMath
 WebWare

JCE Software
   Latest Releases
 Software & Video
 Downloads
 Support

Only@JCE Online
   JCE Online Store
 JCE HS CLIC
 JCE Discussion Forums
   Biographical Snapshots
 ChemEd Resource Shelf
 Featured Molecules
 Hal's Picks
 Project Chemlab
 Reviewed WWW Sites
 "Web-Ed" Articles

About JCE
   Features
 Publications
 Operations
 Outreach
 Contact Us


  Home > JCE Print > Journal of Chemical Education > Issues > 1999  > May  >
In the Laboratory
Concepts in Biochemistry
Monitoring Hammerhead Ribozyme-Catalyzed Cleavage with a Fluorescein-Labeled Substrate: Effects of Magnesium Ions and Antibiotic Inhibitors. A Biochemistry Laboratory: Part 2
Christine S. Chow, Smita Somne, and Beatriz Llano-Sotelo
Department of Chemistry, Wayne State University, Detroit, MI 48202
Cover
May 1999
Vol. 76 No. 5
p. 651
Abstract
An experiment is presented that demonstrates current techniques in modern RNA research and introduces a method for nonradioactive monitoring of RNA reactions. The laboratory involves the study of hammerhead ribozyme activity and the influence of metal ions and antibiotics on these important RNA-based reactions. The ribozyme class of RNA catalysts has current applications in both biotechnology and medicine and therefore should be of great interest to upper-level undergraduate students who anticipate careers in these areas. The students gain hands-on experience in working with RNA on a picomole level and also learn about gel electrophoresis, the use of fluorescent tagging, RNA-small molecule interactions, and the role of metal ions in biological systems. This laboratory offers students an opportunity to work with molecules that have direct applications in drug therapy and RNA catalysis.
Supplement
A student handout with a detailed description of the laboratory experiment is available as supplementary material. The material can be accessed as a pdf file using Acrobat Reader. The experiment is also available as a Microsoft Word document, which has been compressed into a sit (for Macintosh) and a zip (for Windows) file.
*  Contents
*  Download
supp651.pdf

supp651.zip

supp651.sit
More Information
*  Citation
 Chow, Christine S.; Somne, Smita; Sotelo, Beatriz Llano. J. Chem. Educ. 1999 76 651.
*  Keywords
 Biochemistry; Bioorganic Chemistry; Electrophoresis; Nucleic Acids / DNA / RNA; Enzymes; Bioinorganic Chemistry
*  History
 Created:
Last Updated:		 June 11, 1999
November 22, 2005
<< Previous Article Table of Contents Next Article >>
  Home > JCE Print > Journal of Chemical Education > Issues > 1999  > May  > Page 651


Subscriptions
 Subscription Info
 Order Forms

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.
 JCE HS CLIC

Contributions Welcome
JCE welcomes your submission
 Contributors' Corner

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.
 Recent Advertisers

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.
 Outreach with JCE

Comments to
jceonline@chem.wisc.edu		 Copyright © Division of Chemical Education, Inc., American Chemical Society.
All rights reserved.