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  Home > JCE Print > Journal of Chemical Education > Issues > 1999  > November  >
In the Laboratory
Demonstrating Chemical and Analytical Concepts in the Undergraduate Laboratory Using Capillary Electrophoresis and Micellar Electrokinetic Chromatography
Christopher P. Palmer
New Mexico Institute of Mining and Technology, Department of Chemistry, Socorro, NM 87801
Cover
November 1999
Vol. 76 No. 11
p. 1542
Abstract
This paper describes instrumental analysis laboratory exercises that utilize capillary electrophoresis and micellar electrokinetic chromatography to demonstrate several analytical and chemical principles. Alkyl parabens (4-hydroxy alkyl benzoates), which are common ingredients in cosmetic formulations, are separated by capillary electrophoresis. The electrophoretic mobilities of the parabens can be explained on the basis of their relative size. 3-Hydroxy ethylbenzoate is also separated to demonstrate the effect of substituent position on the acid dissociation constant and the effect this has on electrophoretic mobility. Homologous series of alkyl benzoates and alkyl phthalates (common plasticizers) are separated by micellar electrokinetic chromatography at four surfactant concentrations. This exercise demonstrates the separation mechanism of micellar electrokinetic chromatography, the concept of chromatographic phase ratio, and the concepts of micelle formation. A photodiode array detector is used in both exercises to demonstrate the advantages and limitations of the detector and to demonstrate the effect of pH and substituent position on the spectra of the analytes.
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*  Citation
 Palmer, Christopher P. J. Chem. Educ. 1999 76 1542.
*  Keywords
 Analytical Chemistry; Colloids; Chromatography; Electrophoresis; Laboratory Instruction
*  History
 Created:
Last Updated:		 October 12, 1999
November 22, 2005
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