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1999
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In the Laboratory
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A Structure-Function Study of RecA: The Structural Basis for ATP Specificity in the Strand Exchange Reaction
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Julie Gegner, Natalie Spruill, and Leigh A. Plesniak
Department of Chemistry, University of San Diego, San Diego, CA 92110
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November 1999
Vol. 76 No. 11
p. 1562
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| Abstract |
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The terms "structure" and "function" can assume a variety of meanings. In biochemistry, the "structure" of a protein can refer to its sequence of amino acids, the three-dimensional arrangement of atoms within a subunit, or the arrangement of subunits into a larger oligomeric or filamentous state. Likewise, the function of biological macromolecules can be examined at many levels. The function of a protein can be described by its role in an organism's survival or by a chemical reaction that it promotes. We have designed a three-part biochemical laboratory experiment that characterizes the structure and function of the Escherichia coli RecA protein. The first part examines the importance of RecA in the survival of bacteria that have been exposed to UV light. This is the broadest view of function of the enzyme. Second, the students use an in vitro assay of RecA whereby the protein promotes homologous recombination. Because RecA functions not catalytically, but rather stoichiometrically, in this recombination reaction, the oligomeric state of RecA in complex with DNA must also be discussed. Finally, through molecular modeling of X-ray crystallographic structures, students identify functionally important features of the ATP cofactor binding site of RecA.
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| Supplement |
Experimental write-ups and instructor's notes are available.
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| More Information |
Citation |
Gegner, Julie; Spruill, Natalie; Plesniak, Leigh A. J. Chem. Educ. 1999 76 1562.
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Keywords |
Biochemistry; Molecular Modeling / Dynamics; Enzymes; Laboratory Instruction
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History |
Created:
Last Updated: |
October 12, 1999
November 22, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
1999
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November
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1562
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