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  Home > JCE Print > Journal of Chemical Education > Issues > 1999  > November  >
In the Laboratory
Advanced Chemistry Classroom and Laboratory
An in Vivo 13C NMR Analysis of the Anaerobic Yeast Metabolism of 1-13C-Glucose
Brent J. Giles, Zenziwe Matsche, Ryan D. Egeland, Ryan A. Reed, Scott S. Morioka, and Richard L. Taber
Department of Chemistry, The Colorado College, Colorado Springs, CO 80903
Cover
November 1999
Vol. 76 No. 11
p. 1564
Abstract
A biochemistry laboratory experiment that studies the dynamics of the anaerobic yeast metabolism of 1-13C-D-glucose via NMR is described. Fleischmann's Active Dry yeast, under anaerobic conditions, produces primarily 2-13C-ethanol and some 1-13C-glycerol as end products. An experiment is described in which the yeast is subjected to osmotic shock from an increasing sodium chloride concentration. Under these conditions, the yeast increases the ratio of glycerol to ethanol. The experiment can be accomplished in a single laboratory period.
More Information
*  Citation
 Giles, Brent J.; Matsche, Zenziwe; Egeland, Ryan D.; Reed, Ryan A.; Morioka, Scott S.; Taber, Richard L. J. Chem. Educ. 1999 76 1564.
*  Keywords
 Biochemistry; Laboratory Instruction; NMR Spectrometry; Isotopes; Carbohydrates
*  History
 Created:
Last Updated:		 October 12, 1999
June 23, 2005
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