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2000
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August
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Research: Science and Education
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Significant Figures, the Periodic Table, and Mass Spectrometry: The Challenge of Large Biomolecules
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Nancy Carter Dopke, Paul M. Treichel, and Martha M. Vestling
Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706
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August 2000
Vol. 77 No. 8
p. 1065
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| Abstract |
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The number of significant figures required for meaningful mass spectrometric data for
biomolecules is considered. Both resolution and calibration issues are addressed. The
details of the isotopic clusters expected for acetic acid (C2),
bradykinin (C50),
ubiquitin (C378), soybean trypsin inhibitor (C892), and glycogen
phosphorylase b (C4367)
are presented. Although masses are known for isotopes to more than six significant figures,
relative abundance data generally contain fewer than four significant figures, which limits
the number of significant figures that should be reported for large molecules (> 10,000 Da)
and the precision of the periodic table.
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| More Information |
 Citation |
Dopke, Nancy Carter; Treichel, Paul M., Jr.; Vestling, Martha M. J. Chem. Educ. 2000 77 1065.
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Keywords |
Mass Spectrometry; Isotopes; Periodicity / Periodic Table; Proteins / Peptides; Biochemistry
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History |
Created:
Last Updated: |
July 5, 2000
April 15, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
2000
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August
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1065
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