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  Home > JCE Print > Journal of Chemical Education > Issues > 2000  > December  >
In the Laboratory
Turbidimetric Analysis of Water and Wastewater Samples Using a Spectrofluorimeter
Jason J. Evans
Chemistry Department, Dickinson College, Carlisle, PA 17013-2896

Cover
December 2000
Vol. 77 No. 12
p. 1609

Abstract
As student interest in environmental science grows, many colleges and universities are developing new courses in environmental chemistry. Environmental analysis in the "real world" has become increasingly instrumental, and it is important to introduce students to the instruments and procedures that are commonly used in environmental laboratories. Turbidimetric analysis of water and wastewater is ordinarily performed in environmental laboratories using a nephelometer. This experiment illustrates that a spectrofluorimeter can be successfully employed for these types of analysis. Samples from various stages of the water and wastewater treatment processes were collected from the Carlisle Water and Wastewater Treatment Plants. The students in our Environmental Chemistry laboratory used the spectrofluorimeter to measure the scattering intensity from the samples and from a series of formazine standards. The standard curve produced from their data gave a correlation coefficient of .999, and the detection limit was 0.03 Standard Turbidity Units, which is sufficient to obtain meaningful data on most water samples. This experiment was an excellent supplement to lecture material covering water and wastewater treatment because the students were able to monitor the level of suspended particulates in the water as it makes its way through the treatment plants.
Supplement
The lab handout used for this experiment is available.
*  Contents JCE2000p1609W.doc (Microsoft Word)
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JCE2000p1609W.pdf

JCE2000p1609W.sit

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More Information
*  Citation
Evans, Jason J. J. Chem. Educ. 2000 77 1609.
*  Keywords
Analytical Chemistry; Colloids; Environmental Chemistry; Fluorescence Spectrometry; Instrumental Methods; Laboratory Instruction; Water / Water Chemistry
*  History
Created:
Last Updated:
November 3, 2000
August 31, 2005
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