|
|
| Home > JCE Print > Journal of Chemical Education > Issues >
2000
>
March
> |
|
In the Laboratory
|
|
|
|
A Convenient and Highly Specific Western Blot Experiment for Introductory Biochemistry
|
Christopher J. Fenk and Stephanie Y. Grooms
Chemistry Department, Kent State University-East Liverpool Campus, East Liverpool, OH 43920
Donald G. Gerbig Jr.
Biology Department, Kent State University-East Liverpool Campus, East Liverpool, OH 43920
|
|
March 2000
Vol. 77 No. 3
p. 373
|
|
|
|
|
|
|
| Abstract |
|
An experiment is described for use in undergraduate biochemistry laboratories. It is designed to take advantage of recent technological improvements in Western blot procedures and may be conveniently conducted in two consecutive 3-hour laboratory periods using readily available reagents. A commercially available mixture of proteins, SDS-PAGE standards, is employed. This eliminates the need to optimize protein concentration for gel loading and immunological detection. In terms of laboratory safety, only proteins from plants and nonprimate animals are used, thus eliminating any concerns related to employing reagents derived from humans or other primates. Use of precast polyacrylamide gels eliminates the hazards associated with handling acrylamide. A chemically inert blotting membrane is employed in place of the more generally used and highly flammable nitrocellulose membrane. These improvements allow for a safe, rapid, and highly selective laboratory protocol that may be easily incorporated into the undergraduate laboratory.
|
| Supplement |
Objectives, background information, experimental notes, Post Lab questions,
and figures are included.
|
 Contents |
|
| Download |
|
|
| More Information |
Citation |
Fenk, Christopher J.; Grooms, Stephanie Y.; Gerbig, Donald G., Jr. J. Chem. Educ. 2000 77 373.
|
Keywords |
Biochemistry; Laboratory Instruction; Enzymes; Proteins / Peptides; Separation Science; Intermolecular Forces
|
History |
Created:
Last Updated: |
February 14, 2000
August 31, 2005
|
|
|
|
|
|
| Home > JCE Print > Journal of Chemical Education > Issues >
2000
>
March
> Page
373
|
|
|
| JCE HS CLIC |
|
Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.
|
| Contributions Welcome |
| JCE welcomes your submission |
| Advertisers |
| In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first. |
| Be An Ambassador |
| Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants. |
|
