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2002
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In the Laboratory
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Cyclodextrin Inclusion Complexes with a Solvatochromic Fluorescent Probe
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Nicole J. Crane, Rudolph C. Mayrhofer, Thomas A. Betts
Department of Physical Sciences, Kutztown University of PA, Kutztown, PA 19530
Gary A. Baker
Department of Chemistry, State University of New York, Buffalo, NY 14260
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October 2002
Vol. 79 No. 10
p. 1261
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| Abstract |
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The fluorescence emission maximum of 6-propionyl-2-(N,N-dimethylamino)naphthalene, PRODAN, shifts about 150 nm between solutions of cyclohexane and water. The tremendous sensitivity of this fluorescent probe to the polarity of its local solvent environment makes it a suitable probe for assessing cyclodextrin, CD, complexation. Students first establish the solvatochromic nature of PRODAN and then use the changes in the emission spectra upon inclusion in β- or γ-CD to determine stoichiometry and formation constants for the complexes.
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| Supplement |
Instructions for students and notes for the instructor are available as a PDF file. An Excel worksheet is also for calculating CD, PRODAN, and water volumes.
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 Contents |
JCE2002p1261W folder containing:
- JCE2002p1261W.doc (Microsoft Word)
- JCE2002p1261W.xls (Microsoft Excel)
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| Download |
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| More Information |
Citation |
Crane, Nicole J.; Mayrhofer, Rudolph C.; Betts, Thomas A.; Baker, Gary A. J. Chem. Educ. 2002 79 1261.
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Keywords |
Equilibrium; Fluorescence Spectrometry; Instrumental Methods; Laboratory Instruction; Molecular Recognition
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History |
Created:
Last Updated: |
August 29, 2002
March 16, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
2002
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October
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1261
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