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  Home > JCE Print > Journal of Chemical Education > Issues > 2004  > June  >
In the Laboratory
Computer Bulletin Board
Application of a Datalogger in Biosensing: A Reagentless Hydrogen Peroxide Biosensor
Lihua Ma and Martin M. F. Choi
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, SAR, China
Cover
June 2004
Vol. 81 No. 6
p. 862
Abstract
The article describes the use of a datalogger in conjunction with an oxygen sensor to fabricate a hydrogen peroxide biosensor. An eggshell membrane is peeled from a fresh egg. The membrane is used to cover the surface of an oxygen electrode. The detection scheme is based on the increase in dissolved oxygen content upon exposure to hydrogen peroxide solution. The increase in oxygen level is monitored and linearly related to hydrogen peroxide concentration. This biosensor has been successfully applied to determine the hydrogen peroxide concentration in some commercial samples. These experiments provide suitable simple hands-on experience for undergraduate and postgraduate students to help them understand the biosensor technology.
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Instructions for the students and notes for the instructor are available.
*  Contents JCE2004p0862W.doc (Microsoft Word)
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*  Citation
 Ma, Lihua; Choi, Martin M. F. J. Chem. Educ. 2004 81 862.
*  Keywords
 Analytical Chemistry; Biochemistry; Instrumental Methods; Laboratory Computing / Interfacing; Laboratory Instruction; Quantitative Analysis
*  History
 Created:
Last Updated:		 May 2, 2004
January 21, 2005
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