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  Home > JCE Print > Journal of Chemical Education > Issues > 2005  > January  >
In the Laboratory
Lipid Extraction and Cholesterol Quantification: A Simple Protocol
M. Carmo Barreto
Departmento de Ciências Tecnológicas e Desenvolvimento, Universidade dos Açores, 9502 Ponta Delgada, Portugal
Cover
January 2005
Vol. 82 No. 1
p. 103
Abstract
A fast and simple protocol for lipid extraction from egg yolk and walnut is presented. Students learn to extract total lipids from both sources and to assess the quantity of cholesterol in lipid extracts by the Liebermann–Buchard method, in a semi-quantitative way. This experiment is easily done in a two-hour lab period, except for the evaporation of lipid extracts to dryness, which proceeds overnight and is weighed by the students the following day. Although enzymatic methods are currently used to measure cholesterol levels, the method described here has the advantage of being simple and quite inexpensive.
Supplement
Instructions for the students and notes for the instructor are available .
*  Contents JCE2005p0103W.doc (Microsoft Word)
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More Information
*  Citation
 Barreto, M. Carmo. J. Chem. Educ. 2005 82 103.
*  Keywords
 Applications of Chemistry; Bioanalytical Chemistry; Biochemistry; Consumer Chemistry; First-Year Undergraduate / General; Humor / Puzzles / Games; Laboratory Instruction; Lipids; Natural Products; Public Understanding / Outreach; Quantitative Analysis
*  History
 Created:
Last Updated:		 November 29, 2004
December 14, 2004
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