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  Home > JCE Print > Journal of Chemical Education > Issues > 2007  > July  >
In the Laboratory
Analysis and Identification of Acid–Base Indicator Dyes by Thin-Layer Chromatography
Daniel D. Clark
Department of Biology, Chemistry, and Environmental Science, Christopher Newport University, Newport News, VA 23606
Cover
July 2007
Vol. 84 No. 7
p. 1186
Abstract
Thin-layer chromatography (TLC) is a simple and useful technique for the separation of compounds in a mixture. Organic chemists commonly employ TLC to follow the progression of a reaction, to qualitatively analyze a compound or mixture, to determine optimal chromatographic conditions, and to identify the components in a mixture. Additionally, TLC has an established and important role as an educational tool for the introduction of chromatographic principles in many organic chemistry laboratory curricula. Therefore, the development of organic chemistry laboratory experiments, as educational tools, that effectively illustrate chromatographic principles is essential. Presented herein is a TLC experiment that has been developed for the analysis and identification of common acid–base indicator dyes. This experiment serves to illustrate the fundamentals and utility of chromatography through the resolution of brightly colored acid–base indicator dyes and identification of the dyes on the basis of Rf (ratio to front or retention factor) values and color. Additionally, this experiment serves to introduce organic chemistry students to the chemical structures of commonly encountered acid–base indicator dyes and the underlying chemistry for the color changes of these indicator dyes in various pH environments.
Supplement
Detailed student handout with background and procedures, instructor notes with comments for the implementation of this experiment including equipment and reagent requirements, and hazard alerts are available.
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Contents
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JCE2007p1186W.doc (Microsoft Word)
More Information
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Citation
Clark, Daniel D. J. Chem. Educ. 2007, 84, 1186.
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Keywords
Acids / Bases; Dyes / Pigments; Hands-On Learning / Manipulatives; Laboratory Instruction; Organic Chemistry; Second-Year Undergraduate; Thin Layer Chromatography
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History
Created:
Last Updated:
5/29/2007
6/7/2007
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