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  Home > JCE Print > Journal of Chemical Education > Issues > 2008  > January  >
In the Laboratory
Analysis of Peppermint Leaf and Spearmint Leaf Extracts by Thin-Layer Chromatography
Libbie S. W. Pelter, Andrea Amico, Natalie Gordon, Chylah Martin, Dessalyn Sandifer, and Michael W. Pelter
Department of Chemistry and Physics, Purdue University Calumet, Hammond, IN 46323-2094
Cover
January 2008
Vol. 85 No. 1
p. 133
Abstract
In this inquiry-based activity, the usefulness of thin-layer chromatography (TLC) to visualize the difference between spearmint and peppermint is explored. The experiment may be used in any class where TLC is discussed from high school to college. We have used this activity with science majors in an organic chemistry laboratory, with non-science majors in a brewing science class, and in a general science class for elementary education majors. The experiment can be completed in a two-hour period. R-(-)-Carvone and (1R,2S,5R)-(-)-menthol are responsible for the cool and minty sensations associated with these plants. These observable properties and the structural formula of the compounds provide the basis for a good discussion on structure–activity relationships.

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A list of the required equipment and reagents, instructions for instructors, and the student handout are available.
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Citation
Pelter, Libbie S. W.; Amico, Andrea; Gordon, Natalie; Martin, Chylah; Sandifer, Dessalyn; Pelter, Michael W. J. Chem. Educ. 2008, 85, 133.
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Keywords
Biochemistry; First-Year Undergraduate / General; Hands-On Learning / Manipulatives; High School / Introductory Chemistry; Inquiry-Based / Discovery Learning; Laboratory Instruction; Natural Products; Organic Chemistry; Plant Chemistry; Thin Layer Chromatography
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History
Created:
Last Updated:
12/4/2007
12/12/2007
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